Collecting umbilical cord blood

Dear Parents!

Collecting umbilical cord blood is a simple, non-invasive and safe procedure, both for the mother and the newborn.

We would like to give you a short description of the collection process and of what happens next to the priceless stem cells.

Collecting umbilical cord blood

Each childbirth ends with the cutting of the umbilical cord. The blood leaking for the umbilical cord (also a natural process) is collected by a qualified midwife into a special SCB nOvum kit.

The cord blood collection kit has been carefully prepared by our experts in order to safely collect the umbilical cord blood and then transfer it to the SCB nOvum laboratory.

After the child is born, the midwife has only a few minutes to collect the precious stem cells. It is the moment between the cutting of the umbilical cord and the placental expulsion (in some situations the blood is collected after the placental expulsion). A Caesarean section also allows for the collection of cord blood.

This is why it is so important to use the correct collection container, which is the key element of the collection kit, for this procedure.

The containers used by SCB nOvum are produced by the French company MacoPharma and are specifically designed, intended and authorized for use in collecting and preparing umbilical cord blood.

This is reflected in the non-standard size (thickness) of the needle, which reduces the time necessary for collecting the blood from the umbilical vein. These containers are used by banks in France, Great Britain, Germany, Italy and other countries.

Besides the collection container, the kit includes additional elements, necessary for the collection of cord blood: a syringe with a needle to collect blood from the mother for serological testing (WR, antiHCV, HBsAg, antiHBc total, antiHIV), sterile surgical gloves, a surgical mask, a sterile surgical sheet, a temperature reader (used to control the temperature in the kit from the moment of collection to the moment when the preparation process begins) and gels for maintaining a stable temperature within the kit.

Additionally the midwife fills in the documentation (provided with the kit) concerning the collection process.

Before collecting umbilical cord blood we get the mother’s medical history, which then allows our supervising doctor (a haematology and transplant specialist) to qualify her for cord blood collection.

Preparing the cord blood

The blood collected and secured by the midwife is then safely and in a controlled way transported, in a closed and labelled kit, to the SCB nOvum laboratory where it undergoes preliminary test (including cell count and cell viability), qualification and then preparation.

Preparation is a process in which the blood is processed in order to reduce its volume and remove the unnecessary and undesirable elements. At this stage we select the white blood cells containing stem cells.

Our laboratory procedures ensure a very high recovery of white blood cells (85%-95%). This is very important information for parentsconsidering depositing their child’s stem cells, since there are many different ways of preparing cord blood.

As a confirmation of our preparation’s effectiveness, together with the deposit certificate, you receive information confirming the percentage of recovered leucocytes in the frozen sample. you receive information confirming the percentage of recovered leucocytes in the frozen sample. confirming the percentage (%) of recovered leucocytes in the frozen sample.

The preparation process is conducted in a closed, sterile system,which prevents infection before freezing.

The prepared stem cells are then placed in a storage cartridge labelled in the ISBT 128* system and frozen in a special device for controlled freezing of cells.

*The ISBT 128 standard is used for the identification and labelling of blood and its components, as well as human human tissue and organs. The standard was designed by the International Society of Blood Transfusion (ISBT) and accepted by the American Association of Blood Banks (AABB), European Blood Aliance (EBA), Food and Drug Administration (FDA) and European Plasma Fractionators Association (EPFA). The ISBT 128 standard is managed by the International Council for Commonality in Blood Bank Automation (ICCBBA) in the US.


Storing umbilical cord blood

The frozen stem cells are then placed in a cryostat and stored in liquid nitrogen (around -196°C). Around three weeks after your child is born, you receive written confirmation of the positive test results and the deposit having been frozen.

That is how long we need to gather the results (the longest wait is for the blood cultures- around 2-3 weeks). In further correspondence we send you a certificate of the deposit, together with the test results, a protocole of the preparation and other information regarding the frozen sample.

As experts with many years of experience in the area of cryopreservation, in order to avoid loss of viability through technological changes in the temperature of the frozen deposits (Transient Warming Events), which occur when the cryostats are opened for the purpose of adding new cartridges, we, as the only team in Poland , invented and implemented an innovative system of storing cartridges!